The Q-Box AQUA Aquatic Respirometry Package is designed for measurements of metabolic rate (VO2) as reduction in Dissolved Oxygen inside the respirometry chamber with an aquatic animal. Both vertebrates and invertebrates may be studied. Q-Box AQUA uses the principle of intermittent flow respirometry.
Q-Box AQUA Operation
The animal is placed in a respirometry chamber and the decrease in dissolved O2 due to consumption by the animal is measured. Raw dissolved Oxygen data corrected for salinity, temperature and pressure is displayed and used to calculate values of oxygen consumption rate (VO2) in the software. Measurements are made sequentially without removing the animal from the chamber. The chamber is flushed with fresh water between measurements. These intermittent flow measurements of VO2 overcome disadvantages of continuous closed respirometry where dissolved O2 level in the chamber may decrease to a hypoxic level. All of the components are housed in a rugged weather proof case for easy transport and storage.
During an experiment water is circulated through the animal chamber and the flow-through vessel with the optical DO probe by the liquid pump. A 3-way valve controlled by Digital Control Unit (DCU) and the software determines if the system is in circulation or flushing mode. During the circulation mode respiration measurements are made and during flush mode the animal chamber is being flushed with oxygenated water from the water bath. The circulation-flush cycle is repeated until the experiment is terminated. The time of the flush and circulation phase are selected by the user in the software.
mini-AQUA for small samples
For aquatic respirometry measurements of small fish and insect lavae (~1g) Qubit offers mini-AQUA package, a modified version of the Q-Box AQUA with small aquatic respirometer chamber (9ml) and components for smaller total volume system. mini-AQUA package also includes a micro-chamber (1.23ml in volume) for measurements of VO2 in very small samples such as larvae, eggs and juvenile fish less than 0.5g. The micro-chamber is used in the intermittent stop flow mode during measurements and the DO probe is placed directly in the chamber resulting in higher sensitivity and resolution of the system. Contact Qubit Systems for details.
G223, micro-chamber of mini-AQUA package
- Optical DO Probe and Water Bath
- Small Respirometry Chamber (140ml, 3.8cm ID, 15.3cm long)
- Large Respirometry Chamber (660ml, 8.2cm ID, 16.7 cm long)
- mini-AQUA chamber (9ml, 1.6 cm ID, 4.5 cm long)
- micro-chamber (1.23ml, 1.5 cm ID, 0.85cm depth) in mini-AQUA
- Temperature & Salinity Probe for Water Bath
- Liquid Pump (1 LPM or 0.1LPM in mini-AQUA) & 3-Way Valve
- Digital Control Unit for 3-Way Valve Control
- 6 Channel Data Interface
- Data Acquisition Software
- Absolute Pressure Sensor
- Rugged Weatherproof Case
Q-Box AQUA Software
Software: Raw Data Page
Software: Data Calculation Page
Dr. Tyson MacCormak, Biology Department, Mount Alison University, New Brunswick, Canada
“We have been using the Q-Box AQUA respirometry package for a year and have been impressed with the quality and versatility of the system. Although designed for teaching, we have found it to be more than adequate for basic research applications on both fish and cephalopods. The mechanical systems are well designed and easily serviced and the software is intuitive and flexible enough for use in diverse applications. The technicians at Qubit are outstanding; they were able to modify the system to suit our particular needs and they have always been quick to respond when technical support was required. The fact that Qubit ships the system in a protective case was also a big plus for field work!”Testimonial