The Q-Box RP1LP Low Range Respiration Package is designed to measure the metabolic rate of small animals such as insects, reptiles, amphibians, other invertebrates and small mammals in an open-flow or stop-flow gas exchange system. The Q-Box RP1LP Low range Respiration Package can even be used for measurements of CO2 and O2 exchange in colonies of fungi and microorganisms. When combined with Qubit Gas Switching System measurements of respiration rates and RQ are possible on up to 7 organisms (8 channel system including reference).
Open flow measurements of respiration are used for larger, or more active animals, whereas for measurements of very small changes in pO2 such as during respiration of small insects the system may be used in a stop flow configuration. In the latter the flow is stopped for a period of time to allow accumulation of CO2 and decline in O2. With subsequent opening of the flow the gas is swept through the sensors for measurements. The opening and stoppage of the flow throught the chamber with the animal is achieved by manual adjustment of two 3-way valves.
Other potential applications for this package include measurements of metabolism from the gas head space above aquatic suspensions. For example, measurement of yeast fermentation. The Q-Box RP1LP can be used to measure O2 consumption rate and CO2 production rate of virtually any biological system in which these changes in pCO2 and pO2 are in the ranges measured by the analyzers.
Q-Box RP1LP Software
Sample data with a 5 meal worms in an open-flow system:
sample data with a single mealworm in stop-flow system:
Q-Box RP1LP software provides calculation templates for VCO2 (with RQ assumptions), and for VCO2 and VO2 determinations in an open-flow and stop-flow system. The Enhanced Q-Box RP1LP package includes automatic calculations of VCO2 and VO2 during the progress of the experiment.
The Q-Box RP1LP Low Range Respiration Package, can easily be adapted to work as Q-Box CO650 Plant CO2 Analysis Package for measurements of photosynthesis, and respiration in leaves by purchasing a G112 Flow Through Leaf Chamber, Q-A101 Lab Stand, and the A113 LED Light Source. For measurements of respiration in larger or more active animals see the Q-Box RP2LP High Range Respiration Package.
Dr.Amanda R. Falk, Department of Biologu, Center College, Danville, KY
“I just wanted to touch base and thank you again for all your help with our qubit low-range respirometry box. It worked great, the students loved designing experiments with it, and it was the centerpiece of our animal physiology lab last spring.”Testimonial