Z991 FluorPen is a chlorophyll fluorescence measuring device for the lab and the field. This hand-held fluorometer uses high output LEDs to provide a saturating pulse for the measurement of Fv/Fm (QY) and other parameters. The Z991 FluorPen is an accurate, portable option to bulky fiber optic chlorophyll fluorometers, especially when the user wants to screen a large number of plants for specific fluorescence parameters.
Four different versions of FluorPen are available. All measure Ft, QY and kinetic responses such as OJIP, NPQ and Light Curve responses of QY, but have either a built in standard leaf clip (Z991) or are adapted to be used with detachable leaf clips (Z991-D). The Fluorpen can also be purchased with a build in PAR meter for measurements in 400-700nm range (Z991-LM or Z991-LM-D)
- Ft – continuous fluorescence yield in non-actinic light. Ft is equivalent to Fo if the leaf sample is dark-adapted.
- QY – Photosystem II quantum yield. In a dark-adapted leaf this is equivalent to Fv/Fm. In a light-adapted leaf it is equivalent to Fv‘/Fm‘.
- OJIP – Chlorophyll Fluorescence Induction Kinetics (fast transient analysis) is a simple and non-invasive tool to monitor chloroplast function. Provided OJIP analysis is used as sensitive and reliable fast test for the functionality and vitality of photosynthetic system.
- NPQ – Non-Photochemical Quenching. Provided are two predefined NPQ protocols differing in the duration of light exposure and dark recovery phase as well as in the number of intervals between the pulses. It is typically used for quantification of photochemical and non-photochemical quenching in dark-adapted samples.
- Light Curve – Adaptation of Quantum Yield to Several Different Light Levels. There are three predefined Light Curve protocols based on pulse modulated fluorometry differing in number and duration of single light phases and light intensities. Light Curve protocols provide successive measurements of the sample photosynthesis under various light intensities of continuous illumination relating the rate of photosynthesis to photon flux density.
Measuring light power is adjustable by flash duration; actinic light is adjustable up to 1000 µmol photons/m2/s and saturating flashes are adjustable up to 3,000 µmol photons/m2/s.
All FluorPens come with two data transfer options built in USB and Bluetooth and they all have a GPS module built in. The FluorPen operates on Lithium battery that can be easily recharged via USB port.
Z991 FluorPen is available with two style of leaf clip. Standard leaf clip is attached to the device, and the removable leaf clip can be placed on the leaf ahead of time for dark adaptation purposes. An optional feature of the FluorPen is the PAR light meter (LM). When included it is built in on the front panel of the device and it measures photosynthetically active radiation in 400-700nm range in units of umol photons/m2/s. The list of available versions is as follows:
Z991 Standard leaf clip FluorPen
Z991-D FluorPen adapted for use with detachable leaf clips (10 leaf clips included)
Z991-LM Standard leaf clip FluorPen with PAR light meter built in
Z991-D-LM FluorPen adapted for use with detachable leaf clips (10 leaf clips included) and built in PAR light meter
NPQ Protocol Includes Five Measurements in Actinic Light and three measurements during dark relaxation.
NPQ 1 protocol: light duration 60s, 5 pulses; dark recovery duration 88s, 3 pulses
NPQ 2 protocol: light duration 200s, 10 pulses; dark recovery duration 390s, 7 pulses
NPQ_Ln = (FM – FM_Ln) / FM_Ln
NPQ_Lss = (FM – FM_Lss) / FM_Lss
NPQ_Dn = (FM – FM_Dn) / FM_Dn
Formulas Derived From:
R.J. Strasser, A. Srivastava and M. Tsimilli-Michael (2000): The fluorescence transient as a tool to characterize and screen photosynthetic samples. In: Probing Photosynthesis: Mechanism, Regulation and Adaptation (M. Yunus, U. Pathre and P. Mohanty, eds.), Taylor and Francis, UK, Chapter 25, pp 445-483.
Explanation of OJIP Parameters:
- Bckg = background
- F0: = F50µs; fluorescence intensity at 50 µs
- FJ: = fluorescence intensity at j-step (at 2 ms)
- Fi: = fluorescence intensity at i-step (at 60 ms)
- FM: = maximal fluorescence intensity
- FV: = FM – F0 (maximal variable fluorescence)
- VJ = (FJ – F0) / (FM – F0)
- Vi = (Fi – F0) / (FM – F0)
- FM / F0
- FV / F0
- FV/ FM
- M0 or (dV / dt)0 = TR0 / RC – ET0 / RC = 4 (F300 – F0) / (FM – F0)
- Area = area between fluorescence curve and FM (background subtracted)
- Fix Area = total area above the OJIP fluorescence transient – between F40µ and F1s(background subtracted)
- SM = area / FM – F0 (multiple turn-over)
- Ss = the smallest Sm turn-over (single turn-over)
- N = SM . M0 . (1 / VJ) turn-over number QA
- Phi_P0 = 1 – (F0 / FM (or FV / FM)
- Psi_0 = 1 – VJ
- Phi_E0 = (1 – F0 / FM)) . Psi_0
- Phi_D0 = 1 – Phi_P0 – (F0 / FM)
- Phi_Pav = Phi_P0 – (SM / tFM); tFM) = Time to reach FM (in ms)
- ABS / RC = M0 . (1 / VJ) . (1 / Phi_P0)
- TR0 / RC = M0 . (1 / VJ)
- ET0 / RC = M0 . (1 / VJ) . Phi_0)
- DI0 / RC = (ABS / RC) – (TR0 / RC)
FluorPen Data capture screen
NPQ data capture screen
OJIP measurements capture screen
LIght Curve captrue screen